Brain Slice Resources

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Slicing Solutions

• NMDG Slicing Solution Recipe by Peter Klein 

  • Based on Ting et al., 2004 

• Sucrose Slicing Solution from John Huguenard's Lab 

• Agar Backstop Recipe

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Artificial Cerebrospinal Fluids

• ACSF Recipe from John Huguenard's Lab 

• ACSF To Assess Effects of Ketone Bodies 

  • Based On Ma et al., 2007 

• ACSF To Assess Effects of Glucose 

  • Based on Kong et al., 2010

 

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Patch Pipette Internals

• Potassium Gluconate a.k.a. Qian-Quan Sun’s GABAB Internal 

• Cesium Gluconate Internal 

• Cesium Chloride Internal 

  • Based on Nusser & Mody, 2002

 

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Tools

• Mark's Model Cell Tester Worksheet 

• Liquid Junction Potential

  • Liquid Junction Potential Calculator 

  • Liquid Junction Potential Explained by Axon Instruments 

  • Liquid Junction Potential Explained by Neher 

• Auto Mouse Clicker (love it) 

• Neuron Electrophysiology Database

• Need a good ion channel blocker? Try this.

 

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Tutorials & Guides

• Setting Up Clampex for Data Acquisition 

• Creating a Stimulus File for Clamplex 

  • Addendum 

 

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Theory

• The Axon Guide (3rd edition) 

• Capacitance & Access Resistance

 

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Cleanup

• Interface Rig Cleanup Checklist

 

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Manuals

• Patch Scope & Camera 

  • Axio Examiner 

  • HC-Image Live 

• Recording 

  • Digidata 1440A 

  • Multiclamp 700A 

  • Multiclamp 700B 

  • pClamp 10 

• Brain Slice Chamber (BSC1r) 

• Stimulating 

  • WPI Stimulus Isolator A365​

 

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Software

• Clampfit Downloads