Vesicular Release

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Outline

1. Steps of the synaptic vesicle cycle

2. Machinery involved in vesicular release

   • SNARE Proteins

   • Synaptotagmin

   • Calcium

3. Modern Perspectives

   • Exocytosis & Endocytosis of Transmitter

   • Molecular Machinery

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Slides

• Vesicular Release

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Reading For Class

• Kandel: Chapters 12. Focus on sections:

  • Transmitter Is Stored & Released by Synaptic Vesicles

  • Exocytosis of Synaptic Vesicles Relies on a Highly Conserved Protein Machinery

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Extra Reading

• Alabi & Tsien, 2013: Perspectives on Kiss-and-Run: Role in Exocytosis, Endocytosis, and Neurotransmission

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• Sudhof, 2013: Neurotransmitter Release: The Last Millisecond in the Life of a Synaptic Vesicle

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• Sudhof, 2013: A molecular machine for neurotransmitter release: synaptotagmin and beyond

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• Ricardo Miledi and the calcium hypothesis of neurotransmitter release